Guanidinoacetic Acid as a Precursor of Creatine for Cattle

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Determination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methylguanidine in biological fluids.

The non-specific analytical methods for the guanidinium compounds in biological fluids have been unsatisfactory for many years. The methods presented in this paper have a greatly improved specificity and are not too complex for use in the clinical laboratory. A modified Sakaguchi color reaction for substituted guanidines is used in each procedure. Guanidinoacetic acid is measured by developing ...

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Creatine Metabolism and Safety Profiles after Six-Week Oral Guanidinoacetic Acid Administration in Healthy Humans

OBJECTIVES Guanidinoacetic acid (GAA) is a natural precursor of creatine, yet the potential use of GAA as a nutritional additive for restoring creatine availability in humans has been limited by unclear efficacy and safety after exogenous GAA administration. The present study evaluated the effects of orally administered GAA on serum and urinary GAA, creatine and creatinine concentration, and on...

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Guanidinoacetic Acid and Creatine are Associated with Cardiometabolic Risk Factors in Healthy Men and Women: A Cross-Sectional Study

Guanidinoacetic acid (GAA) conversion to creatine is thought to be involved in cardiometabolic disturbances through its role in biological methylation and insulin secretion. We evaluated the association of serum GAA and creatine with cardiometabolic risk factors in a cohort of 151 apparently healthy adults (82 women and 69 men) aged 18-63 years. Serum levels of GAA and creatine were measured wi...

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A new enzymic determination of guanidinoacetic acid in urine.

We developed and evaluated a colorimetric method for enzymic determination of guanidinoacetic acid (GAA) in urine. Endogenous urinary urea was first eliminated by urease (EC 3.5.1.5), and the added urease was then removed from the sample by centrifugal ultrafiltration. GAA in the ultrafiltrate was subsequently hydrolyzed by guanidinoacetate amidinohydrolase (EC 3.5.3.2) to glycine and urea. The...

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ژورنال

عنوان ژورنال: Kansas Agricultural Experiment Station Research Reports

سال: 2015

ISSN: 2378-5977

DOI: 10.4148/2378-5977.1150